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Fig. 6. Expression of category III (co-polymerization defect) and category IV (no biochemical defect) mutants in NIH3T3 fibroblasts. I64N incorporated into stress fibers, localized with the endogenous actin and was observed in aggregates and long rods in the cytoplasm, which did not stain with phalloidin (A; scale bar, 25 µm). D286G incorporated into stress fibers in some cells (B) but their structures were wavy (B, inset). D286G also formed aggregates in the cytoplasm that did not stain with phalloidin (C). (A-C) Red indicates Myc staining and green phalloidin staining. (A) The nuclei were stained with Hoechst and appear blue.





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