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Fig. 7. Rod formation by expression of V163L (category II), H40Y (category IV) and I357L (category III) in NIH3T3 fibroblasts. V163L did not localize to normal actin structures but recruited endogenous actin to aberrant structures (A-A'',B-B'', arrows). In the nucleus, it formed aggregate star structures (A-A''; scale bar, 25 µm). Some aggregates were stained with phalloidin but did not detectably contain the mutant actin (B-B'' arrows). H40Y did not localize to normal actin structures but formed rods and aggregates in the nucleus and cytoplasm (C-C'' and not shown). I357L (D-D'') incorporated into stress fibers and lamellipodia and also formed cytoplasmic aggregates, which did not localize with endogenous actin. (A'-D') Red indicates Myc staining and green phalloidin staining. The nuclei were stained with Hoechst and appear blue.
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