|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 2. Antibody ligation of CD9 induces apoptosis in MKN-28 cells. MKN-28 cells were cultured on chamber slides for 96 hours in the presence of 50 µg ml–1 IgG1 (A,C,E,G,I) or ALB6 (B,D,F,H,J). The cultured cells were then fixed with 4.0% paraformaldehyde for 20 minutes, permeabilized with 0.1% Triton X-100 at 4°C for 2 minutes and subjected to staining with rhodamine-phalloidin (A,B), DAPI (C,D) or TUNEL (E,F). Scale bars, 50 µm (A-D), 200 µm (E,F). The cultured cells were harvested, stained with propidium iodide and their nuclear contents analysed by flow cytometry (G,H). The cultured cells were harvested and stained with annexin-V (shaded bars). Control staining is also shown (open bars) (I,J). Similar results were obtained in three independent experiments.
|
