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Fig. 5. TG2 is required for cell spreading. (A) Phase contrast images of fibroblasts cultured on top of collagen I lattices at different time points after seeding. (B) F-actin (FITC-phalloidin) and vinculin (TRITC immunolabelling) distribution in fibroblasts 6 hours after seeding on collagen I-coated glass coverslips. Images represent an extended focus view of five sections of a Z-series taken at 3 µm distance using confocal laser scanning microscopy. The area of the cell shown at high magnification has a blue frame in the insets. In the TG2-deficient cell, the lower inset shows a basal section with vinculin labelling only. Arrows indicate mature focal adhesions associated with stress fibres; arrowheads indicate focal complexes at cell periphery. Scale bars: 100 µm (A) and 10 µm (B).





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