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Fig. 6. Altering TG2 expression affects cell migration in an in vitro wound assay. (A) Confluent fibroblast monolayers were scratch wounded and repopulation of the denuded area followed over a 48-hour period using time-lapse microscopy (see Movies 1-4, http://jcs.biologists.org/supplemental/). (B) The migration paths of 10 cells were tracked over the first 20 hours using the Kinetic Imaging Lucida 4.0 software and the tracks overlaid (using an identical starting point) to illustrate the differences in migration profiles. A representative track is highlighted in colour. (C) An individual cell is highlighted in colour to illustrate the morphological changes in migrating cells. The line (arrowhead) indicates the position of the original edge of the wound. (D) 8 hours after wounding, F-actin was visualised with FITC-phalloidin. A lamellipodium (arrows) is formed in normal and TG2-overexpressing fibroblasts but not in TG2-deficient cells. Scale bars: 100 µm (A), 50 µm (C), and 45 µm (D).





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