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Fig. 7. TG2-deficient cells hyper-phosphorylate FAK but fail to activate PKC ${alpha}$ . Mock (lane 1), TG2 antisense (lane 2) and TG2 sense (lane 3) transfected HCA2 fibroblasts were extracted and equal amounts of protein were separated in 4-20% SDS-PAGE gels. (A) At the indicated timepoints after seeding, cells were analysed for pTyr (top), FAK (middle), and pTyr³⁹⁷ FAK (bottom) by immunoblotting with specific antibodies. Molecular mass standards (kDa) are indicated on the left. (B) Serum starved fibroblasts were extracted after 30 minutes in suspension or at the indicated timepoints after seeding on uncoated or collagen I-coated (ColI) dishes or uncoated dishes in the presence of serum (FCS) and analysed for FAK, pTyr, and Tyr³⁹⁷, Tyr⁵⁷⁶ or Tyr⁹²⁵ phosphorylated FAK. (C) Cytosolic (top) and membrane (bottom) fractions were probed with antibodies to PKC ${alpha}$ .

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