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Fig. 8. PKC activity is required for cell spreading. Phase contrast images (A-J) or vinculin immunolabelling (K,L) of fibroblasts 1 hour after seeding: mock transfected cells under normal culture conditions (A) or after treatment with 10 nM bisindolylmaleimide I (B), 10 nM bisindolylmaleimide V (C), 30 µM PD98059 (D), 1 nM TPA (E), 5 µM U-73122 (F) or 5 µM U-73343 (G); TG2-deficient cells under normal conditions (H,K) or after treatment with 1 nM TPA (I,L) or 5 µM U-73122 (J). Arrows indicate spread and polarized cells (I) and mature focal adhesions (L), respectively. Scale bars: 50 µm (A) and 9.5 µm (K).
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