QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 2. Phenotypic characterization of Med-MO, IL-4-DC and GM-IL-4-DC. (A) Maturation was induced by stimulation with TNF-a (20 ng/ml) for 2 days. Large cells were gated. The fluorescence of gated cells is depicted in the histograms. Dotted lines in each panel represent staining with isotype-matched irrelevant control Igs. Each row of histograms represents FACS profiles of one individual side-by-side comparative experiments. The differentially expressed molecule is CD1a. The expression of CD1a is barely detectable in IL-4-DC compared with GM-IL-4-DC. Each marker was probed in at least three separate experiments. (B) Neutralization of GM-CSF by anti-GM-CSF mAb inhibits CD1a expression of GM-IL-4-DC. Freshly purified monocytes containing >90% CD14⁺ cells (top panel; dotted line represents staining with isotype-matched control Ig, unbroken line represents staining with anti-CD14 mAb) were stained for surface expression of CD1a and GM-CSF receptor chain (CD116) immediately and after culture in media alone, or media containing GM-CSF (800 U/ml) plus IL-4 (1000 U/ml) and graded concentrations of neutralizing anti GM-CSF mAb (1.0 to 10.0 µg/ml), or media containing IL-4 alone. Data shown are from three separate representative experiments.

Return to article