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Fig. 4. Comparative cytofluorographic analysis of phagocytic activity, and intracellular myeloperoxidase (MPO) of freshly isolated and cultured monocytes. Cells were incubated with 3 µM FITC-latex beads for 30 minutes at 37°C (A) or stained with anti-MPO mAb after permeabilization (B) and examined by flow cytometry. Dotted lines in the histograms of MPO represent staining with isotype-matched irrelevant control Ig. Data shown are representative of three separate experiments.





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