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Fig. 5. Detection of cytokine transcripts in IL-4-DC by RT-PCR. Cells were left unstimulated or stimulated with LPS (1 µg/ml) for 6 hours, total RNA were extracted and were analysed for expression of mRNA for IL-12, IL-10, IFN-{gamma}, TNF-{alpha}, and ß-actin. Data are representative of three similar experiments.





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