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Fig. 2. dsRNA binding activity of NRF. Uniformly 32P-labeled 469 nt IFN-{gamma} T7-derived RNA (see Methods section) was incubated with in vitro produced full-length NRF fusion protein or the indicated deletion mutants. The reaction mixture was subjected to electrophoresis on a native gel to separate RNA-protein complexes from the free probe. The arrows indicate the migration of IFN-{gamma} RNA as a free probe and as complexes with NRF and PKR. The autoradiogram shows free and bound RNA. Addition of antibody directed against the Myc-tag (that specifically inhibits the complex formation between His-Myc-NRF and IFN-{gamma}) is indicated.





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