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Fig. 5. FLIP analysis of NRF-GFP. C243 cells were stably transfected with NRF-GFP and subjected to FLIP analysis. The bleached regions in the cytoplasm (A) or in the nucleoplasm (B) are indicated with white rectangles and fluorescence intensity is shown in false color code. Each image series shows the fluorescence prior to bleaching (0 seconds) and after three consecutive bleaching periods of the indicated time (34 seconds for A; 27 seconds for B).





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