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Fig. 7. Quantitative FRAP analysis of NRF-GFP during viral infection. C243 cells expressing full-length NRF-GFP or the nucleolus marker protein GFP-hfbr2_82i24 were infected with Sendai virus or Newcastle disease virus (NDV). Before and at the indicated time points post infection cells were subjected to FRAP analysis as described in Fig. 6A. Inter-nucleolic mobility of NRF-GFP was measured. For clarity, only fluorescence recovery kinetics for the bleached nucleoli are shown. The change of fluorescence intensity in the unbleached nucleoli were also determined and do not differ from the changes presented in Fig. 6A. Each individual time point represents the average of data from five single cells. (A) NRF-GFP-expressing cells infected with Sendai virus. (B) NRF-GFP-expressing cells infected with NDV. (C) GFP-hfbr2_82i24-expressing cells infected with Sendai virus.





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