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Fig. 1. (A) Distribution of HMGA1a-GFP fusion proteins in transfected cells. (a) Distribution of HMGA1a-GFP in living cells as revealed by fluorescence microscopy. Corresponding phase contrast image is shown in a'. (b) Optical section of a living cell expressing HMGA1a-GFP and overlay of the fluorescence picture with the corresponding dichromatic picture (b'). The distribution of the fusion proteins (c) is comparable with the distribution of endogenous HMGA1 in non-transfected cells (c') and overlaps with endogenous HMGA1 in transfected cells (c''). (B) Distribution of HMGA1a-GFP overlaps with Hoechst DNA staining in situ (a,a') and in formaldehyde-fixed cells (b,b'). Bars correspond to 10 µm.





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