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Fig. 2. (A) Co-localization of HMGA1a-GFP and histone H1. HMGA1a-GFP pattern is shown in (a), histone H1 distribution in (a') and the merged picture in (a''). (b-b'') Localization of HMGA1a-GFP compared with that of nascent transcripts. Nascent transcripts were BrUTP-labeled by in situ run-on transcription. After fixation, incorporated BrUTP was visualized by immunofluorescence (b'). (c-c'') Co-localization of HMGN2-GFP with nascent transcripts (c'). Merged picture is shown in c''. Most right panels are magnifications of the boxed areas in a'', b'' and c'', respectively. Bars correspond to 10 µm. (B) Electron microscopy of untransfected (a,a') or HMGA1a-GFP expressing cells (b,b'). Higher magnifications of the boxed areas in (a) and (b) are shown in (a') and (b'), respectively. HMGA1a-GFP was localized with secondary antibodies coupled to 12 nm gold particles (b', arrows). Note that HMGA1a expression does not alter bulk chromatin structure on ultrastructurel level (compare a and b) and that HMGA1a-GFP proteins are concentrated in domains (arrows, b'). Bars represent 5 µm in (a,b) and 200 nm in (a',b').

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