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Fig. 5. (A) Fluorescence recovery after photobleaching (FRAP) of cells expressing HMGA1a-GFP. Cells were bleached for 1 second in an area of approximately 1 µm (circle) and the recovery of fluorescence was measured in interphase (arrows, upper panels) or mitotic cells (arrows, lower panels). Pictures of selected time points of fluorescence recovery are shown as indicated. Bars represent 10 µm. (B-D) Quantitative analyses of FRAP experiments in euchromatin (B; compared with heterochromatin, hc), in heterochromatin (C; compared with heterochromatin near nucleolus and heterochromatin in nucleoplasm) and in chromosomes (D; compared with heterochromatin). Note the difference in the kinetic properties after bleaching heterochromatin near the nucleolus or within the cytoplasm (C) and the reduced mobility of HMGA1a-GFP bound to chromosomes (D). (E) Comparison of recovery kinetics with members of the HMGB- and the HMGN-families.





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