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Fig. 6. In vivo distribution (A) and dynamics (B) of fusion proteins with point mutations in the AT hook DNA-binding motifs. The mutants contained a substitution of the second arginine by glycine in the consensus AT hook peptide PRGRP. GFP-fusion proteins analyzed contained a single point mutation within AT hook I (R28G), II (R60G), III (R86G), two point mutations in AT hooks I and II (R28,60G), I and III (R28,60G), II and III (R60,86G) or three point mutations in AT hook I, II and III (R3xG). (A) Distribution of mutated fusion proteins in interphase (upper panels) or during mitosis (lower panels). Pictures are optical sections made with a confocal laser scanning microscope. Note the increased homogeneous distribution in interphase and the reduced chromosomal localization during mitosis compared with the wild-type fusion protein (wt). (B) Recovery kinetics of the point-mutated proteins introduced in (A) as revealed by FRAP.

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