QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 2. Annexin 2 is recruited to PtdIns(4,5)P₂-rich structures elicited by active Arf6. (A) HeLa cells, transiently expressing wild-type Arf6 or co-expressing Arf6 and PHD-YFP, were treated for 30 minutes with AlF (30 mM NaFl and 50 µM AlCl₃) and processed for annexin 2 immunostaining or plasma membrane labeling with CM-DiI, respectively. Both annexin 2 (anx2) and PHD-YFP localize to plasma membrane protrusions induced by AlF-mediated Arf6 activation (arrowheads). To ascertain that increased signals at sites of membrane ruffling are not owing to a general increase in membrane thickness, mean fluorescence intensity ratios for signals in ruffled (arrowheads) to non-ruffled (arrows) regions were determined for PHD-YFP and DiI, respectively. Results are presented as percent-ratio of the mean fluorescence intensity ± s.e.m. ^*P<0.05, bars 10 µm. (B) HeLa cells were co-transfected with constitutively active Arf6 Q67L and PHD-YFP, GFP-actin, annexin 2 (anx2)-GFP, or YFP-p11. Annexin 2-GFP and YFP-p11 both clearly localize to the GFP-actin-positive, PtdIns(4,5)P₂-rich vacuoles that were induced by Arf6 Q67L expression. Bars 10 µm.

Return to article