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Fig. 1. Chk1 kinase activity is rapidly induced following UV irradiation and remains elevated for the duration of the checkpoint. (A) Checkpoint response of wild-type (chk1:ep) G2 cells to 150 J m–2 UV-C. Cells were synchronized by centrifugal elutriation and then irradiated ({blacksquare}) or left untreated ({square}) (t=0). The proportion of cells either passing mitosis or with septa and cell number was determined. Chk1 kinase activity was assayed at the time points indicated. Data shown is representative of three independent elutriations. (B) Phosphorylated Chk1 (Chk1) was detected by western blotting. (C) Asynchronous wild-type cells (chk1:ep) were irradiated with 0 J m–2, 25 J m–2, 50 J m–2, 75 J m–2 or 150 J m–2 UV-C, and extracts were prepared 30 minutes after irradiation. The average Chk1 kinase activity from three concurrent assays is shown and error bars represent standard error. Chk1 and hyperphosphorylated Chk1 (Chk1) was detected by immunoblotting with anti-HA antibodies





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