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Fig. 2. chk1-ts1 is a conditional allele of chk1 that is active at 25°C but not at 36°C. (A) UV-C and ionizing radiation survival curve for wild-type (chk1:ep) ({square}), chk1{Delta} ({blacksquare}) and chk1-ts1 ({circ}) at 25°C and 36°C. Strains were grown at the temperature shown and irradiated with the indicated dose of UV-C (J m–2). Data are normalized against unirradiated controls, and error bars represent s.e.m. (n=3-9). (B) MMS sensitivity assay for chk1:ep, chk1{Delta} and chk1-ts1. Strains were grown at the temperatures indicated then serial dilutions of culture inoculated onto YES agar plates containing 0.01% MMS. Plates were incubated at the specified temperature to allow colony formation.





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