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Fig. 3. chk1-ts1 is conditionally checkpoint defective. (A) Checkpoint assays for asynchronously growing wild-type (chk1:ep) ({square}), chk1D ({blacksquare}) and chk1-ts1 ({circ}) at 25°C and 36°C. Cells were irradiated with 150 J m–2 UV-C and septation indices counted at each time point. Data are normalized against the time of irradiation (t=0). (D) DAPI-stained chk1-ts1 cells from both the 25°C and 36°C asynchronous time courses, with or without UV-C irradiation. Cells showing aberrant mitotic figures are indicated by the arrows. Scale bar, 10 µm.





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