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Fig. 4. chk1-ts1 kinase activity is induced following irradiation at 25°C but not at 36°C. (A) Comparison of kinase activity of asynchronous wild-type (chk1:ep) and chk1-ts1 cells grown at 25°C or 36°C and irradiated with 150 J m–2 UV-C or 150 Gy ionizing radiation. Samples were taken 10 minutes, 20 minutes and 30 minutes after irradiation. Error bars represent standard errors. (B) Chk1 and hyperphosphorylated Chk1 (top) was detected by immunoblotting with anti-HA antibodies.





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