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Fig. 7. Chk1 is required for UV-C-induced checkpoint maintenance. (A) Wild-type (cdc10-M17chk1:ep) cells were grown to mid-logarithmic phase at 25°C and then shifted to 36°C for 4.75 hours, after which the cultures were shifted to 25°C. Half of the culture was irradiated with 50 J m–2 UV-C ({blacksquare}) and the other half mock irradiated ({square}). After an additional 20 minutes at 25°C, one irradiated and one mock-irradiated culture were shifted to 36°C, with duplicate cultures left at 25°C. Samples were taken at the indicated times and cells passing mitosis assayed by DAPI staining. (B) Repeat of the experiment in (A) but using cdc10-M17chk1{Delta} cells. (C) Repeat of the experiment in (A) but using cdc10-M17chk1-ts1 cells.





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