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Fig. 2. Identification of a C-terminal NLS. (A) GFP was fused to the N terminus of full-length chicken ETR-3 variant L (GFPcETR3vL) and deletion constructs were subsequently made removing the last 58 (427-484) or 20 (465-484) amino acids from the C terminus, producing truncated proteins with molecular masses of 72.5 kDa or 76.6 kDa (GFPcETR3 ${Delta}$ and GFPcETR3 ${delta}$ , respectively). These C-terminal fragments were also fused to the C terminus of a GFP-PK fusion construct to produce chimeric proteins with molecular weights of 81.8 kDa (GFP-PK- ${Delta}$ ) or 77.7 kDa (GFP-PK- ${delta}$ ). (B) GFPcETR3vL is nuclear. The C-terminal deletion constructs GFPcETR3 ${Delta}$ and GFPcETR3 ${delta}$ , in contrast, are predominantly cytoplasmic. The localization of GFP-PK is shifted to the nucleus by the addition of the C-terminal 58 or 20 amino acids of ETR-3 (GFP-PK- ${Delta}$ and GFP-PK- ${delta}$ , respectively). Scale bar: 10 µm.

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