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Fig. 3. Identification of nuclear localization and export elements in the divergent domain. (A) The divergent domain of ETR-3 variant L was divided into four parts (residues 188-240, 241-293, 294-346, and 347-399) and deletions of each of these four segments were made within GFPcETR3vL (GFPDD.1-4) and GFPcETR3 ${Delta}$ (GFPDD.1 ${Delta}$ -4 ${Delta}$ ). (B) Western blot analysis using an anti-GFP antibody confirmed that all expression plasmids expressed proteins of the expected sizes, and the deletion mutants all expressed protein at comparable or greater levels than the full-length protein. (C) Deletion of the fourth divergent domain region alone (GFPDD.4) resulted in loss of nuclear localization in transfected cells, indicating this region is required for nuclear localization. Deletion of the first and second divergent domain regions in conjunction with the C terminus (GFPDD.1 ${Delta}$ and GFPDD.2 ${Delta}$ ) resulted in partial restoration of nuclear localization, indicating the presence of sequences required for cytoplasmic localization. Scale bar: 10 µm.

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