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Fig. 4. A region of the divergent domain is sufficient for cytoplasmic localization in primary embryonic chicken cardiomyocytes. (A) The first three quadrants of the divergent domain (residues 188-346) of ETR-3 variant L were fused to the NLS-GFP-PK chimeric protein (NLS-GFP-PK-DD.123). (B) Western blot analysis using an anti-GFP antibody confirmed that proteins of the expected sizes were produced from both expression plasmids. (C) The NLS-GFP-PK chimeric protein is normally nuclear (top panels). Addition of the first three quadrants of the divergent domain resulted in cytoplasmic localization in transfected primary embryonic chicken cardiomyocytes (bottom panels). Scale bar: 10 µm.





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