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Fig. 7. Identification of regions important for cytoplasmic localization in RRM1 and RRM2. (A) Deletions within the first two RRMs of ETR-3 (residues 1-89 and 90-178, respectively) were made within GFPcETR3vL (GFPRRM.1-2) and GFPcETR3 ${Delta}$ (GFPRRM.1 ${Delta}$ -2 ${Delta}$ ), producing truncated proteins with molecular masses of 68.9 kDa (GFPRRM.1), 69.0 kDa (GFPRRM.2), 62.4 kDa (GFPRRM.1 ${Delta}$ ), and 62.6 kDa (GFPRRM.2 ${Delta}$ ). (B) Deletions in RRM1 and RRM2 alone had no effect on localization, but deletions made in conjunction with the deletion of the NLS at the C terminus resulted in partial nuclear localization, indicating sequences within the first two RRMs are important for cytoplasmic localization of ETR-3. Scale bar: 10 µm.

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