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Fig. 2. Jasplakinolide treatment accentuates microvilli growth on elongating bristle tips. (a) Thin section through the apical portion of an untreated newly emerging bristle shaft cell (B) from a 33-hour pupa. A portion of the neuron (N) that innervates the bristle is present in this section. Note that pimples are present extending from the surface of this emerging bristle shaft cell (arrowheads) as well as from the surface of the adjacent socket cell (SC). (b-d) 33-hour pupal thoraces were cultured in jasplakinolide for 1 hour before fixation and thin sections of emerging tips are shown here. In b-d microvilli are seen extending from the bristle tips. In panels c and d (which are adjacent sections) microvilli have extended from the pimples from adjacent epithelial cells (E). (e) Higher magnification of the microvillus indicated by the squares in panels c and d. As these are adjacent sections we have pasted the squares together to show the entire length of the microvillus. Within this microvillus one can easily see the core of actin filaments. There are transverse stripes on this core bundle (lines) which indicate the presence of crossbridges between the filaments.
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