QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 3. ₂ß₁^hi/CD133 population have a high proliferative potential in vitro. (A) Basal cells with the phenotypes ₂ß₁^hi/CD133⁺ and ₂ß₁^hi/CD133^- were plated onto type I collagen plates and the colony forming efficiency (CFE) determined. Cells were counted before the addition of irradiated feeders and were cultured for up to 28 days. Controls (basal cells) were plated with no pre-selection. Colonies containing 32 or more cells were scored. CFE was calculated as the number of colonies formed per number of selected cells x100. CFEs are expressed as the ratio of the selected population to control (unselected basal cell) population. Results show means ± s.e.m. of four experiments. (B) Long-term proliferative potential of ₂ß₁^hi/CD133⁺ and ₂ß₁^hi/CD133^- cells. 5x10³ cells were seeded onto type I collagen plates, in triplicate, and the total cell output was determined at the end of the serial culture when their growth capacity was exhausted. Results show means ± s.e.m. of four experiments. P<0.05.

Return to article