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Fig. 2. The alternative RF-C^{Ctf18/Dcc1/Ctf8}, CHL1 and CTF4 are required for chromosome segregation during meiosis and for spore viability. Diploid wild-type (K8409), ctf18 ${Delta}$ (K10488), dcc1 ${Delta}$ (K10117), ctf8 ${Delta}$ (K10092), chl1 ${Delta}$ (K11603), ctf4 ${Delta}$ (K11681) and trf4 ${Delta}$ (K12384) strains in which both homologs of chromosome V were marked by GFP (homozygous URA3-GFP) were sporulated and chromosome segregation in tetrads was scored under the fluorescence microscope. Spore viability (n=100) was determined by dissection. Diploid wild-type (K10003), ctf18 ${Delta}$ (K10608), dcc1 ${Delta}$ (K10352), ctf8 ${Delta}$ (K10576), chl1 ${Delta}$ (K11652), ctf4 ${Delta}$ (K11692) and trf4 ${Delta}$ (K12661) strains in which only one copy of chromosome V was marked by GFP (heterozygous URA3-GFP) were sporulated and chromosome segregation in tetrads was scored under the fluorescence microscope. More than 200 tetrads of each strain were scored for URA3-GFP segregation. n.d., not determined.

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