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Fig. 3. (A-D) CTF8 is required for sister-chromatid cohesion during meiosis. Diploid wild-type (K10003) (A,B) and ctf8 ${Delta}$ (K10576) (C,D) strains expressing Pds1-Myc18 (securin) and carrying one homolog of chromosome V marked by GFP (heterozygous URA3-GFP) were analysed in a meiotic time course experiment. Cells were fixed and stained for DNA and with antibodies against tubulin and the Myc epitope. Fluorescence microscopy images of various meiotic cell cycle stages (indicated below the images) of wild-type (A) and ctf8 ${Delta}$ (C) cells showing URA3-GFP (green), tubulin (red) and DNA (blue) are arranged in the upper panels as a simulated time course. The lower panels in (A,C) display the securin staining of the cells above them. Fluorescent-microscopy pictures of a sporulated wild-type (B) and ctf8 ${Delta}$ (D) tetrad stained for DNA (blue) and showing heterozygous URA3-GFP (green). (E,F) Deletion of CHL1 does not cause frequent meiosis I non-disjunction of homologues. Diploid wild-type (K8409) (E) and chl1 ${Delta}$ (K11603) (F) strains carrying both homologs of chromosome V marked by GFP (homozygous URA3-GFP) were analysed in meiotic time course experiment. Cells were fixed and stained for DNA and with antibodies against tubulin. Wild-type (E) and chl1 ${Delta}$ (F) anaphase I cells displaying URA3-GFP (green), tubulin (red) and DNA (blue) are shown. For each strain, 100 anaphase I cells were scored in this experiment.

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