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Fig. 5. Deletion of CTF8, CHL1 and CTF4 rescues the nuclear division block of mam1 ${Delta}$ cells in anaphase I. Diploid mam1 ${Delta}$ (K8923) (A,B) and mam1 ${Delta}$ ctf8 ${Delta}$ (K10572) (D,E) strains expressing Pds1-Myc18 (securin) and carrying one homolog of chromosome V marked by GFP (heterozygous URA3-GFP) were analysed in a meiotic time course. Cells were fixed and stained for DNA and with antibodies against tubulin and the Myc epitope. Fluorescent-microscopy images of securin-positive metaphase I cells with a bipolar spindle (A,D), a securin-negative `abortive' anaphase I (B) and a securin-negative elongated anaphase I (E) are shown. (A,B,D,E) The upper panels show URA3-GFP (green), tubulin (red) and DNA (blue); the lower panels show securin staining (white) of the cells above them. Schematic drawings illustrating an `abortive' anaphase of mam1 ${Delta}$ cells (C) and the rescued anaphase in mam1 ${Delta}$ ctf8 ${Delta}$ cells (F) showing meiotic cohesin in red and URA3-GFP in green. (G) The percentage of nuclear division and spindle elongation in wild-type (K10003), ctf8 ${Delta}$ (K10576), chl1 ${Delta}$ (K11652), ctf4 ${Delta}$ (K11692), mam1 ${Delta}$ (K8923), mam1 ${Delta}$ ctf8 ${Delta}$ (K10572), mam1 ${Delta}$ chl1 ${Delta}$ (K11703) and mam1 ${Delta}$ ctf4 ${Delta}$ (K11828) cells following the destruction of securin in anaphase I. 100 securin-negative cells with a bipolar metaphase I or anaphase I spindle were scored for each strain in this experiment.

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