QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 2. Gnu phosphorylation and expression. (A) Immunoblot with anti-GFP antibody demonstrating the slow-mobility, phosphorylated form of GnuGFP in ovaries from transgenic w; P{w⁺ gnuGFP}GG4C/+; gnu females (lanes O). Only the dephosphorylated form was found in their embryos (lane E) and in w; P{w⁺ gnuGFP S/T>A}/+; gnu ovaries from lines DF4, BM1, EM1 and GM3 (lanes 1-4 respectively) containing independent heterozygous insertions of the transgene. (B,C) Confocal sections of (B) w; P{w⁺ gnuGFP}GG4C/+; gnu/+ and (C) w; P{w⁺ gnuGFP S/T>A}DF4/+; gnu/+ ovaries showing distribution of GnuGFP (green) and DNA (red). Expression levels of wild-type and mutant Gnu were similar. However, GnuGFP from the wild-type w; P{w⁺ gnuGFP} transgene was not detected at stage 6 and was noticeably above w[1118] background (not shown) only in stages 9 and 10. By contrast, mutant Gnu from the P{w⁺ gnuGFP S/T>A} transgene was detectable at stage 6, accumulated in nurse cells but relatively little was found in stage 10 oocyte before the dumping of nurse cell contents.

Return to article