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Fig. 7. Stabilisation and loss of cyclin B in a Gnu gradient. (A) cyclin B in a w; gnu P{w⁺ gnuGFP-bcd3'UTR}T1 (Gnu gradient) embryo. (B) Merged images of cyclin B (green), ${alpha}$ -tubulin (blue) and DNA (red). (C,D) Detail of threshold region at approximately 25% egg length. (C) Metaphase ${alpha}$ -tubulin (blue), DNA (red). (D) Metaphase spindle-associated and cytoplasmic cyclin B. (E) Posterior region of Gnu gradient embryo showing loss of phosphorylated histone H3 (green) on anaphase chromosomes (DNA: red). Compare with the wild type in the inset F. (G) Posterior region of Gnu gradient embryo stained for centrosomal ${gamma}$ -tubulin (green), spindle ${alpha}$ -tubulin (blue) and DNA (red). The posterior-most mitotic nucleus is tetraploid, with four centrosomes organising a tetrahedral spindle array. Scale bar: $~$ 10 µm.

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