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Fig. 1. The Adphr-EGFP adenoviral vector. (A) The marsupial CPD-photolyase gene (gene phr) was fused with EGFP in its C-terminal. Thereafter, the phr-EGFP expression cassette, under the control of the cytomegalovirus (CMV) promoter and with the bovine growth hormone (BGH) poly(A) signal, was cloned in the pAdeno-X vector by standard recombinant technology at the indicated restriction sites. This plasmid was transfected into low passage HEK 293 cells for the production of recombinant viral particles containing the phr-EGFP gene. (B) The integrity of the phr-EGFP insert in the recombinant adenovirus vector: the presence of the sequences phr, EGFP and poly(A) signal regions from the virus vector were confirmed by a PCR reaction, with the positive controls pCY4Bphr, pEGFP-N1 and pAdeno-X, respectively.





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