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Fig. 2. Photorepair protects Adphr-EGFP-infected cells from UV irradiation. (A) Infection of primary cells with Adphr-EGFP vector. Normal human primary fibroblasts (198VI cell line) were infected with Adphr-EGFP (m.o.i. 1.6x10⁵) and the fluorescence of EGFP was analyzed after 40 hours by fluorescence microscope (photos of representative fields; 60x objective) and flow cytometry of cell suspension (graphics). For the graphics of fluorescence, `counts' represents the number of cells and `FL1-H' the green fluorescence intensity. M1 corresponds to the percentage of EGFP fluorescence calculated (CellQuest Software, Becton Dickinson, USA). (B) Survival of XP12RO-SV cells after UV irradiation. Approximately 6.0x10⁵ cells were mock-infected (dotted lines, squares) or infected with the Adphr-EGFP vector (m.o.i. 4x10⁴; unbroken lines, triangles) and, after 40 hours, sub-cultivated in low density for analysis of clonogenic capacity. The cells were UV-irradiated at the indicated doses and immediately exposed to dark (closed symbols) or PRL (open symbols) conditions The bars indicate standard errors (s.d.; each sample in triplicate, two independent experiments).

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