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Fig. 3. CPD immunostaining in UV-irradiated 198VI and XP456VI cells. 5.0x104 cells were infected with Adphr-EGFP adenovirus and, 40 hours later, the cells were exposed to UV irradiation (10 J/m2). Different samples were maintained under dark or PRL conditions during 2 hours immediately after a period of 4 hours incubation. Thereafter, the cells were fixed and immunostained for CPD as described in the Materials and Methods. The cells were analyzed in fluorescence microscopy (Zeiss Axiovert S100, Hamamatsu 3CCD color camera). Representative fields of each sample for fluorescein and TRITC filters are shown.





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