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Fig. 6. Redistribution of phr-EGFP after PRL exposure in primary cells. (A) Normal human primary fibroblasts (198VI cell line) infected with Adphr-EGFP were local UV irradiated with 50 J/m2, immediately exposed to dark or PRL conditions and analyzed after different periods of time. In the representative photos analyzed by fluorescence microscopy, cells locally UV irradiated and kept in dark conditions display strongly-fluorescent foci of phr-EGFP in damaged sites (foci pattern A, indicated by blue arrows). Following PRL conditions, foci of phr-EGFP with less intense fluorescence (foci pattern B, indicated by yellow arrows) in comparison with cells kept in dark for the same period of time. (B) Normal human primary cells (198VI cell line), infected with Adphr-EGFP were locally UV irradiated with 50 J/m2 and immediately exposed to PRL during 2 hours. Four hours after local UV irradiation the cells were incubated with an anti-CPD. Two rows of representative fields in fluorescence microscopy are shown (60x objective).





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