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Fig. 8. Quantification of phr-EGFP fluorescence in foci sites in relation to non-irradiated areas. Normal (A; 198VI), XPA (B; XP456VI) and XPC mutated (C; XP148VI) human primary fibroblasts infected with Adphr-EGFP were locally UV irradiated with 50 J/m2. The cells were exposed to dark (black bars) or PRL (white bars) treatments immediately after local UV irradiation and then analyzed after the indicated periods (1 hour and 4 hours). The intensity of green fluorescent signal in UV-irradiated areas (foci) was quantified using the Molecular Analyst software (BioRad, CA) for a population of 50 cells (198VI and XP456VI cell lines) and 10 cells (XP148VI cell line) and compared to non-irradiated areas in the same nucleus.





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