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Fig. 3. Actin binding of CaD39 or CaD39-AB. Recombinant protein CaD39 at 2.01 µM, 4.02 µM, 4.47 µM, 4.92 µM (A) or CaD39-AB at 2.68 µM, 5.37 µM, 5.82 µM, 7.76 µM (B) was incubated with 9.3 µM F-actin in 10 mM imidazole buffer (pH 7.0) containing 30 mM KCl, 0.1 mM EGTA and 1 mM DTT. After incubation and subsequent centrifugation, both supernatants and pellets were adjusted to equivalent volumes and analysed by SDS-PAGE. `S' and `P' represent the supernatant (free) and pellet (bound) fraction, respectively, of CaD in each reaction. The binding data from three independent experiments were quantified using Scion Image software (C), which showed no significant difference of actin-binding ability between CaD39 and CaD39-AB.
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