spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 5. Effect of PD98,059 on the stretch-induced attenuation of mRNA expression of C/EBP{delta} and PPAR{gamma}. The 3T3-L1 cells were incubated in induction medium (DEX/MIX/INS) containing either 20 µM PD98,059 (designated PD) or 0.01% DMSO (Vehi) for 3 hours and 45 hours with or without cyclic stretching. The total RNA of 3T3-L1 cells at different times during the respective induction periods was subjected to quantitative RT-PCR and blots of the PCR products were hybridized with DIG-labelled cDNA probes corresponding to each target mRNA. (A) Representative results of the hybridization experiment for quantitatively amplified PCR products. The results of C/EBP{delta} at 3 hours as well as PPAR{gamma}2 and PPAR{gamma}1 at 45 hours after the onset of the induction period are shown. (B-D) Summarized results of the quantitative RT-PCR analysis of the effect of PD98,059 on the expression of C/EBP{delta} (B), PPAR{gamma}2 (C) and PPAR{gamma}1 (D). The white bar represents the cells before induction (Pre), the black bar represents the cells without stretching (Rest) and the grey bar represents the cells with cyclic stretching (Stretch; at 130%, 1 Hz) at 3 hours or 45 hours of induction (n=4 each). Abbreviations: Pre, before induction; Vehi, 0.01% DMSO; PD, 20 µM PD98,059. *P<0.05; **P<0.01.





Right arrow Return to article