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Fig. 6. Western-blot analysis of the PPAR{gamma} protein. (A) Specificity of western-blot analyses using anti-PPAR{gamma} antibody. Total cellular proteins (20 µg) from undifferentiated (Pre) and differentiated (Mat) 3T3-L1 cells were analysed by western blot using anti-PPAR{gamma} antibody. (B) Representative western blot and (C) summarized densitometric analyses of PPAR{gamma} expressed in the differentiating 3T3-L1 cells with or without the stretching in the presence or absence of PD98,059. The 3T3-L1 cells were incubated in induction medium (DEX/MIX/INS) containing either 20 µM PD98,059 (designated PD) or 0.01% DMSO (Vehi) for 45 hours with or without cyclic stretching. The black bar represents the cells without stretching (Rest) and the grey bar represents the cells with cyclic stretching (Stretch; at 130%, 1 Hz) during the induction period (n=4 each). *P<0.05; **P<0.01.





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