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Fig. 7. Effect of PD98,059 on the stretch-induced inhibition of adipocyte differentiation of 3T3-L1 cells. (A) Phase-contrast microscopic images of 3T3-L1 cells at 45 hours after incubation in induction medium containing either20 µM PD98.059 or 0.01% DMSO with or without cyclic stretching (a-d), and the images of Oil-Red-O-stained 3T3-L1 cells after a maturation period of 9 days (e-h). (a,e) Unstretched cells incubated with 0.01% DMSO during the induction period. (b,f) Stretched cells incubated with 0.01% DMSO during the induction period. (c,g) Unstretched cells incubated with 20 µM PD98,059 during the induction period. (d,h) Stretched cells incubated with 20 µM PD98,059 during the induction period. (B) GPDH activity. (C) Intracellular triglyceride accumulation after maturation period of differentiated 3T3-L1 cells with (grey) or without (black) cyclic stretching (130%, 1 Hz), in the presence or absence of 20 µM PD98,059 during the induction period (n=4 each). Abbreviations: PD, 20 µM PD98,059; Vehi, 0.01% DMSO; DEX/MIX/INS, induction medium containing DEX, MIX, INS and FBS; ^*P<0.05; ^**P<0.01.

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