spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 2. Identification of a nucleolar localization signal. (A) Mutation of the basic amino acids in the region 143-146 (RKKR) to alanine residues prevents localization of YFP-NIK1-794 in nucleoli while still retaining its nuclear accumulation. Moderately expressing cells were analyzed by confocal microscopy. (B) Fusion of the seven basic amino acids 143-149 of NIK to either the C terminus of GFP (GFP-RKKRKKK) or the N terminus of GFP (RKKRKKK-GFP) induces accumulation of the modified GFP in nucleoli. An additional accumulation in the nucleoplasm is observed for the C-terminally tagged GFP-RKKRKKK.





Right arrow Return to article