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Fig. 6. FRAP analysis to determine diffusion and mobility of NIK variants within a compartment. (A) Mobility of NIK in cytosol (cyt.), nucleoplasm (nuclear) and nucleoli (nucleolar) as determined by FRAP. Variants of YFP-NIK with the respective major localization were analyzed for fluorescence recovery after photobleaching as described in the Materials and Methods section. The values for the calculated maximum recovery (max) indicating the fraction of mobile molecules and the calculated halftime (t1/2) of diffusion are specified. Representative curves are shown. Mean values of halftimes normalized for the bleaching area and standard deviations are specified in the text. (B) Mobility of nucleolar YFP-NIK compared to the GFP-chimera of the ribosomal protein L23 and nucleolar GFP. Halftimes of diffusion and maximum values are given.





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