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Fig. 1. Immunolocalization of EhCaBP1 in erythrophagocytosing E. histolytica. Trophozoites grown for 48 hours were transferred to pre-warmed, acetone-washed cover-slips for 10 minutes at 37°C. The attached cells were further incubated with RBC for 7 minutes. The cells were then fixed with paraformaldehyde/PBS, permeabilized with 0.1% Triton X-100/PBS followed by immunostaining with various antibodies mentioned below. Amoebae were double labeled for EhCaBP1 and (A) F-actin (Phalloidin Red), (B) myosin IB and (C) PAK. Myosin IB and PAK were labeled with CY3-conjugated secondary antibody and EhCaBP1 with Alexa-green secondary antibody. Scale bar: 5 µm. Arrow indicates the region of enriched co-localization. Notice co-localization (yellow) of EhCaBP1 with F-actin and myosin IB around the phagocytic cup and with PAK in the pseudopod.





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