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Fig. 3. Distribution of F-actin is affected in tetracycline-induced EhCaBP1-AS cells during phagocytosis. The attached EhCaBP1-AS (A) and EhCaBP1-AS+tet (B) cells were incubated with RBCs for 7 minutes prior to fixing and immunostaining. Amoebae were double labeled for EhCaBP1 and F-actin (Phalloidin red). EhCaBP1 was labeled with Alexa-green secondary antibody. Notice the actin rich phagocytic cup in the EhCaBP1-AS cell (arrow), absence of a cup around the RBC (arrow) and reduced expression of EhCaBP1 in EhCaBP1-AS+tet cell.