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Fig. 4. Distribution of F-actin and EhCaBP1 in jasplakinolide- and cytochalasin D-treated cells. The HM-1: IMSS cells grown for 48 hours were incubated at 37°C with jasplakinolide at 10 µM for 30 minutes or cytochalasin D at 10 µM in a reaction volume of 1 ml for 15 minutes or with DMSO alone. The cells were pelleted and fixed before immunostaining with rhodamine-phalloidin (red) and anti-EhCaBP1 antibody. EhCaBP1 was labeled with Alexa-green secondary antibody. Finally the cell-pellet was diluted in DABCO for laying on glass slides. Notice enrichment of EhCaBP1 in areas where F-actin was fixed by jasplakinolide. Scale bar: 5 µm.
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