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Fig. 1. Inhibition of transport at 15°C. Cells were transfected with plasmid DNA encoding cargo proteins and incubated at 15°C for 2 hours prior to imaging. ts045-G-CFP (A) colocalises with lumYFP (B) (arrowheads) whereas procollagen-CFP (D) is localised to different discrete structures when compared with lumYFP (arrows) (E). GPI-CFP (G and J) colocalises with both ts045-G-YFP (H) and lumYFP (K). C,F,I,L are merged images of the respective images in the same row. Bars, 10 µm.





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