QUICK SEARCH:

[advanced]

	Author:		Keyword(s):

Home Help Feedback Subscriptions Archive Search Table of Contents

	Help viewing high resolution images
	Return to article

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 6. Confirmation of tyrosine phosphorylation and surface localisation of erp99 and hsp60 on mouse spermatozoa. (A) Cauda epididymal spermatozoa were either freshly isolated (lanes 1 and 3) or capacitated for 90 minutes in BWW–Ca²⁺+Sr²⁺+ptx+cAMP (lanes 2 and 4). CHAPS detergent lysates (5 µg protein/lane) (lanes 1 and 2) and purified surface-protein extracts from 100 µg lysate (lanes 3 and 4) were prepared, resolved by SDS-PAGE and immunoblotted with anti-hsp60 and anti-erp99 monoclonal antibodies. (B) Cauda epididymal spermatozoa were capacitated for 90 minutes in BWW. Proteins were solubilised, resolved by two-dimensional electrophoresis and immunoblotted with anti-phosphotyrosine (a), anti-erp99 (b) and anti-hsp60 (c) antibodies.

Return to article